Toll-free:400-690-0195 English 中文版
   
control for siRNA
In order to get correct analysis experimental data, a suitable control experiment is necessary. A fluorescence-labeled siRNA negative control, which has no genetic homology with human, is conducive to the optimization of transfection conditions, and also can be used as the non-specific silencing control.
 

In order to get correct analysis experimental data, a suitable control experiment is necessary. A fluorescence-labeled siRNA negative control, which has no genetic homology with human, is conducive to the optimization of transfection conditions, and also can be used as the non-specific silencing control. 
SiRNA positive control is the known siRNA which can highly effectively silence specific gene, and it can be used in conventional parallel control experiment to ensure the optimized experimental conditions and the effect of gene silencing.
GenePharma provides convenient positive and negative siRNA controls for human and mouse cells RNAi experiments. The characteristics of siRNA control are as follows:

  • We provide a complete set of experimental controls, which can be used to optimize the RNAi experimental conditions in human and mouse cell lines.
  • Special RNAi-mate transfection reagent is provided to ensure the highly efficient transfection;
  • Transfection efficiency can be monitored easily by fluorescence-labeled negative control;
  • The effect of gene silencing can be identified flexibly by follow-up experiments such as quantitative PCR or western blot.

1. Negative Control
A negative control should be included in a completed siRNA experiment. During the siRNA primary screening, you may choose our custom negative control, which has no homologous sequence with the target gene. 
In order to do further study of gene function, the negative control siRNA should have the same composition as the selected siRNA sequences, but have no obvious homology with mRNA. The general method is to disorder the selected siRNA sequence, and to determine results simultaneously to ensure there is no homology with other genes in target cells.

2. Fluorescent dye-labeled siRNA Negative Control 
GenePharma RNAi negative control has no homology with mammalian gene. After labeled by fluorescent dye, the negative control can be easily observed under fluorescent microscope to get the transfection efficiency, and is helpful for optimizing transfection conditions. The fluorescence labeled control can be easily photographed, and has great PH tolerance, so that it is much stable in living cell.

Cat. No.

Production

Quantity

Purification

Price

Appropriate Times

A06001

negative control siRNA

1 OD

HPLC

$ 29

2business days

A07001

FAM labeled negative control siRNA

1 OD

HPLC

$ 38 

2business days

3. SiRNA Positive Control
Positive control is very important for the inspection of an experimental system. In other words, when you see the expected results of siRNA positive control, you can ensure that your transfection, RNA extraction and detection methods are reliable.

Popular siRNA Positive Control

1

LaminA/C

2

GFP274

3

Luciferase GL2

4

MAPK1

5

Beta-Actin

6

Vimentin

7

P53

8

GAPDH

9

Cyclophilin B

Cat. No.

Production

Quantity

Purification

Price

Times

A0800X

positive control siRNA

1 OD

HPLC

$29

4 business days

 4 Mock Transfection
For a complete control system, mock transfection is essential. Mock transfection can detect the toxicity of transfection reagent towards cells, the survival rate of cells, and other factors of cell transfection.
We recommend the use of our GenePharma transfection reagent - RNAi-Mate. (See Transfection Reagent part)

 Cat. No.

Production  

Quantity

Price

Times

C-01

RNAi-Mate Transfecion Reagent

0.1mL

$26

4 business days

 
Description
Experimental
Usage
Application
Ordering