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miRNA detection kit
The Hairpin-itTM miRNAs qPCR Quantitation Kit is a sensitive and specific method using real-time PCR for the detection and quantification of miRNAs (miRNA) from total RNA samples.
 
The Hairpin-itTM miRNAs qPCR Quantitation Kit is a sensitive and specific method using real-time PCR for the detection and quantification of miRNAs (miRNA) from total RNA samples. MiRNAs are small, single-stranded, ~19–23 nt RNA molecules encoded in the genomes of plants, animals, and viruses. Mature miRNAs enter the RNA-induced silencing complex (RISC) and guide the RISC to induce translational repression or endonucleolytic cleavage of specific target mRNAs. Unlike commonly used methods for detection of miRNAs, the Hairpin-itTM miRNAs qPCR Quantitation Kit is more rapid and sensitive. 


This Kit contains miRNA specific RT and PCR primer set, with a Molecular Beacon probe included. The stem-loop like miRNAs RT primer and the miRNAs specific Beacon probe ensure the RT and PCR reaction would not be interfered by the miRNAs precursors. The reaction template could be total RNA or cell lysates.


To normalize for RNA content among different experimental samples, the U6 snRNA qPCR Normalization Kit (Cat # QPM-050) and 5S rRNA qPCR Normalization Kit (Cat # QPM-060) are available separately.
 
Hairpin-itTM miRNAs qPCR Quantitation Kit Reagents box includes the following items. Store the components at –20℃. Reagents must be stored in the dark.


Reagent
Amount
Storage
 
100rxns
200rxns
500 rxns
 
Real-time PCR Master Mix(5×)*
1.0 ml
2×1.0 ml
5×1.0 ml
4℃
miRNA specific primer Set(10µM)
100μl
200μl
1.0 ml
-20℃
miRNA RT primer(10µM)
15μl
50μl
100μl
-20℃
Taq DNA polymerase (5U/µl)
50μl
100μl
200μl
-20℃
Synthetic miRNA Standard
1nmol
 
 
-20℃
1X RNA Dilution buffer
2×1.0 ml
4×1.0 ml
10×1.0 ml
4℃
*Include dNTP Mixture,Mg2+, Molecular Beacon probe.
 
Accessory Products

Some of the reagents supplied in the Hairpin-itTM miRNAs qPCR Quantitation Kit as well as other products suitable for use with the kit are available separately from Genepharma. Ordering information is provided below.
Item
Amount
Catalog no.
U6 snRNA qPCR Normalization Kit
20 rxns
50 rxns
QPM-090
QPM-091
5S rRNA qPCR Normalization Kit
25μl×50 rxns
25μl×100 rxns
QPM-080
QPM-081
miRNAs qPCR Quantitation Core Kit
100 rxns
200 rxns
500 rxns
QPM-030
QPM-031
QPM-032
Custom miRNAs Quantitation Kit
40µl×50 rxns
40µl×100 rxns
40µl×200 rxns
QPM-040
QPM-041
QPM-042
 
Description
Application
Ordering