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shRNA vector
The siRNA expression vector with antibiotics labeling can continuously suppress the target gene expression, lasting for several weeks or even longer
 

The siRNA expression vector with antibiotics labeling can continuously suppress the target gene expression, lasting for several weeks or even longer.
When a validated siRNA sequence (for example, obtained by screening in siRNA synthesis method) needs to maintain gene silencing for a longer period of time, we recommend the use of shRNA vector system. GenePharma is committed to providing the most advanced and convenient shRNA related tools. The company recently introduces a new generation of shRNA expression plasmid, a highly efficient ready to use vector, which can produce shRNAs sustainedly in cell, so as to achieve lasting suppression of target gene expression.

GenePharma SuperSilencing shRNA Expression Vector System

Characteristics of shRNA Expression Vector System.

  1. Produces short-hairpin RNA (shRNA) to achieve RNA interference.
  2. The method is simple, economical, and only needs to synthesize DNA oligos.
  3. The use of cloning vector is convenient, and the vector has selectable markers.
  4. shRNA plasmid is stable and easy to operate.

GenePharma SuperSilencing shRNA Expression Vector characteristics:

  1. The cloning vector has two cloning restriction sites BamH I and Bbs I. Bbs I is a special restriction endonuclease; it can produce asymmetrical complementary sticky ends to ensure the correct direction of inserted fragments, and to prevent the self-circularization of vectors.
  1. Multiple screening markers can help to establish stable transfected cell lines.

Neo:  Neomycin resistance gene. 
Hygro: Hygromycin B resistance gene.
GFP: Neo: GFP report gene and the Kan/G418 resistance gene.

GenePharma can construct eight kinds of RNAi vectors.
pGPU6, pGPH1, pGPU6/Neo, pGPH1/Neo, pGPU6/Hygro, pGPH1/Hygro, pGPU6/GFP/Neo, pGPH1/GFP/Neo.
pGPU6, pGPH1 is the RNAi vector without screening marker. 
pGPU6/Neo, pGPH1/Neo is the RNAi vector with Neomycin resistance screening marker. pGPU6/Hygro, pGPH1/Hygro is the RNAi vector with Hygromycin resistance screening marker.
pGPU6/GFP/Neo, pGPH1/GFP/Neo is the RNAi vector with Neomycin resistance and GFP report gene expression.


GenePharma SuperSilencing shRNA Expression Vector Pack
Each pack contains 4 shRNA vectors designed for a target gene; we guarantee that at least one of them can cause more than 70% inhibition efficiency in mRNA level.

Pack composition

Number

shRNA vector for a target gene

4

positive control shRNA vector

1

negative control shRNA vector

1

Attention:Each plasmid in this pack is 50ug.



Product

Price

Times

SuperSilencing shRNA Expression Vector Pack


2 weeks

GenePharma SuperSilencing shRNA vector system 
Each set includes linear or cyclic shRNA expression vectors, the corresponding negative control vector and a GFP positive control vector. We also provide you with the overall solution.

Cat. No.

Product

Promoter

Selection marker

Quantity

Price

E-01

pGPU6

U6

6*50μg

$650

E-02

pGPH1

H1

6*50μg

$650

E-03

pGPU6/Neo

U6

Neo

6*50μg

$650

E-04

pGPH1/Neo

H1

Neo

6*50μg

$650

E-05

pGPU6/Hygro

U6

Hygro

6*50μg

$650

E-06

pGPH1/Hygro

H1

Hygro

6*50μg

$650

E-07

pGPU6/GFP/Neo

U6

Neo

6*50μg

$650

E-08

pGPH1/GFP/Neo

H1

Neo

6*50μg

$650

GenePharma ready-to-use SuperSilencing shRNA Expression Vector Cloning and Construction Service 
We can help you to insert the DNA fragment encoding target shRNA into the plasmid and do sequence validation. You just need to tell us the target gene sequence or Gene ID and the name of the inserted vector, and we will help you to construct the expression vector. Furthermore, we will provide you with sufficient purified expression plasmid encoding your target shRNA. 

Cat. No.

Product

Promoter

Selectable Marker

Quantity

Price

Times

C02001

pGPU6

U6

50μg

$149

2 weeks

C02002

pGPH1

H1

50μg

$149

2 weeks

C02003

pGPU6/Neo

U6

Neo

50μg

$149

2 weeks

C02004

pGPH1/Neo

H1

Neo

50μg

$149

2 weeks

C02005

pGPU6/Hygro

U6

Hygro

50μg

$149

2 weeks

C02006

pGPH1/Hygro

H1

Hygro

50μg

$149

2 weeks

C02007

pGPU6/GFP/Neo

U6

Neo

50μg

$149

2 weeks

C02008

pGPH1/GFP/Neo

H1

Neo

50μg

$149

2 weeks

 
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