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RT-PCR kits
Real-time quantitative PCR (RT-qPCR) is an innovative and reliable technique for quantitative analysis of gene expression, mutation detection, allele discrimination and single nucleotide polymorphisms (SNP) genotyping.
 

Real-time quantitative PCR (RT-qPCR) is an innovative and reliable technique for quantitative analysis of gene expression, mutation detection, allele discrimination and single nucleotide polymorphisms (SNP) genotyping. Real-time quantitative PCR was developed to overcome the basic weakness of the classical PCR technology: whereas PCR can indicate the presence of a particular nucleic acid in a sample after a completed PCR amplification, it cannot directly quantify the amount of amplification.
Real-time quantitative PCR systems determine the amount of amplified product by measuring the quantity of a fluorescent dye. This dye, upon excitation, emits a light or signal that increases in intensity in direct proportion to the amount of amplified PCR product and, therefore, quantifies the amount of target DNA. The assays can be directly performed in a sealed tube without any purification or separation step, avoiding all risks of contamination and post-PCR handling.
Real-time quantitative PCR systems also enable mutation detection via melting-curve analysis, as each double-stranded DNA product has its own specific melting temperature (Tm). Melting-curve data enables the researcher to differentiate between specific PCR products and non-specific PCR products, such as primer-dimers.

 Sequence-specific Probes and Non-Sequence-Specific Probes
Detection by real-time quantitative PCR can be sequence-specific or non-sequence specific, and the method chosen will affect the performance of your real-time quantitative PCR results. For instance, SYBR® Green 1, a non-sequence-specific reagent, can bind to any double-stranded DNA including primer-dimers and other non-specific reaction products, and so may lead to an over-estimation of the amount of target DNA. However, sequence-specific probes, such as Taqman probe, dual-labeled fluorescence probe, or Molecular Beacons, bind only to the target template and therefore achieve accurate and reliable quantification of the amount of target DNA.

Design and Synthesis Service of RT-PCR Primers 
GenePharma provides design of primer or probe for free. 
We design primers by using of optimized primer design techniques and latest database resources, simultaneously utilizing all kinds of bioinformatics knowledge and skills to select the best primers. You may get the best experimental results by using of primers and RT-PCR reagents provided by our company in our recommended standard reaction conditions.

 Service for primer design has following characteristics:

  1. Not easy to form primer-dimer;
  2. Primers are normally designed crossing exon junction, which makes it hard to amplify genomic DNA;
  3. The sequence of primer does not contain known SNP site (Confirmed by dbSNP);
  4. The specificity is confirmed through homology search;
  5. Choosing the design region under experimental purposes;
  6. Normally, 1 - 3 pairs of corresponding primers are provided for one target gene.
  7. Design according to the full sequence of target gene;
  8. Design among the 1,500 bases at 5’ end;
  9. Design among the 1,500 bases at 3’ end.

Customer must provide the following information: 

  1. Biology species information (such as Human, Mouse, Rat);
  2. Target gene information (GeneBank Acc, GeneID, key word);
  3. Primer design region (full sequence, 5 ' end sequence, 3 ' end sequence);
  4. Primers, including SYBR Green I, Taqman probes and molecular beacons primers.

Cat. No.

Probe Type

Purification

Quantity

Price

Times

5’ reporter

3’ quencher

P-01

FAM

DABCYL

PAGE+HPLC

2 OD

$99

1 week

P-02

FAM

TAMRA

PAGE+HPLC

2 OD

$99

1 week

P-03

FAM

BHQ-1

PAGE+HPLC

2 OD

$108

1 week

P-04

TAMRA

BHQ-2

PAGE+HPLC

2 OD

$108

1 week

P-05

HEX

DABCYL

PAGE+HPLC

2 OD

$133

1 week

P-06

Cy3

DABCYL

PAGE+HPLC

2 OD

$158

1 week

P-07

TET

DABCYL

PAGE+HPLC

2 OD

$116

1 week

P-08

JOE

DABCYL

PAGE+HPLC

2 OD

$133

1 week

P-09

ROX

BHQ-2

PAGE+HPLC

2 OD

$133

1 week


 
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