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miRNA RT-PCR detection kits
The Hairpin-itTM miRNAs qPCR Quantitation Kit is a sensitive and specific method using real-time PCR for the detection and quantification of miRNAs (miRNA) from total RNA samples.
 

Hairpin-itTM miRNAs qPCR Quantitation Kit

The Hairpin-itTM miRNAs qPCR Quantitation Kit is a sensitive and specific method using real-time PCR for the detection and quantification of miRNAs (miRNA) from total RNA samples. MiRNAs are small, single-stranded, ~19–23 nt RNA molecules encoded in the genomes of plants, animals, and viruses. Mature miRNAs enter the RNA-induced silencing complex (RISC) and guide the RISC to induce translational repression or endonucleolytic cleavage of specific target mRNAs. Unlike commonly used methods for detection of miRNAs, the Hairpin-itTM miRNAs qPCR Quantitation Kit is more rapid and sensitive. 

This Kit contains miRNA specific RT and PCR primer set, with a Molecular Beacon probe included. The stem-loop like miRNAs RT primer and the miRNAs specific Beacon probe ensure the RT and PCR reaction would not be interfered by the miRNAsprecursors. The reaction template could be total RNA or cell lysates.

To normalize for RNA content among different experimental samples, the U6 snRNA qPCR Normalization Kit (Cat # QPM-050) and 5S rRNA qPCR Normalization Kit (Cat # QPM-060) are available separately.

Hairpin-itTM miRNAs qPCR Quantitation Kit Reagents box includes the following items. Store the components at –20.Reagents must be stored in the dark.

Reagent

Amount

Storage

100rxns

200rxns

500 rxns

Real-time PCR Master Mix5×)*

1.0 ml

2×1.0 ml

5×1.0 ml

4

miRNA specific primer Set10µM

100μl

200μl

1.0 ml

-20

miRNA RT primer10µM

15μl

50μl

100μl

-20

Taq DNA polymerase 5U/µl

50μl

100μl

200μl

-20

Synthetic miRNA Standard

1nmol

-20

1X RNA Dilution buffer

2×1.0 ml

4×1.0 ml

10×1.0 ml

4

*Include dNTP MixtureMg2, Molecular Beacon probe.

Accessory Products

Some of the reagents supplied in the Hairpin-itTM miRNAs qPCR Quantitation Kit as well as other products suitable for use with the kit are available separately from Genepharma. Ordering information is provided below.

Item

Amount

Catalog no.

U6 snRNA qPCR Normalization Kit

20 rxns

50 rxns

QPM-090

QPM-091

5S rRNA qPCR Normalization Kit

25μl×50 rxns

25μl×100 rxns

QPM-080

QPM-081

miRNAs qPCR Quantitation Core Kit

100 rxns

200 rxns

500 rxns

QPM-030

QPM-031

QPM-032

Custom miRNAs Quantitation Kit

40µl×50 rxns

40µl×100 rxns

40µl×200 rxns

QPM-040

QPM-041

QPM-042

 
Description
Application