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miRNA agomir & antagomir
Antagomir is a type of specially
 

Antagomir is a type of specially labeled and chemically-modified single-stranded microRNA, designed based on the mature microRNA sequence, which is special for inhibiting the expression of endogenous microRNA. Agomir is a type of specially labeled and chemically-modified double-stranded microRNA,it can regulate the biological function of target gene by mimicking endogenous microRNA. GenePharma could provide the upgraded product of miRNA mimics, inhibitors: miRNA agomir and antagomir.

The Advantages of Antagomir and Agomir:

  • Compared with common miRNA inhibitor and mimics, both antagomir and agomir have a higher affinity for cell membrane, in the cell transfection experiment, the amount of transfection reagent could be drastically reduced.
  • Especially suitable for animal in vivo RNAi experiment and have higher stability and inhibiting effect, can be injected into the animal by the methods of intravenous or local injection, easy to operate.
  • It can be richened in target cells, inducing effective, specific and stable interfering effect.
  • The interfering effect can last a long time, at least one week; the longest interfering effect can be up to 5-6 weeks.

The Chemically-modified Construction of Antagomir and Agomir
Antogimir: 3’ terminal was modified by cholesterol, there are two thiols modification in the 5’ terminal, four thiols modification in the 3’ terminal, and the whole strand is modified by 2’-OMe.
Agomir: in the antisense strand, 3’ terminal was modified by cholesterol, there are two thiols modification in the 5’ terminal, four thiols modification in the 3’ terminal, and the whole strand is modified by OMe.

 

 

Experimental result comparison

 

 

(Fig. a) It shows the interfering eff ect of antagomir miRNA is much better than other chemical modif ied miRNA.

(Fig. b) Antagomir can inhibit the target microRNA specif ically. The effects of antagomir -122 were found to be specific because a control for antagomir -122 that harboured f our mismatch mutations (mm- antagomir-122) had no effect on miR-122 levels. Furthermore, expression levels of let7 and miR-22 were unaffected treated with antagomir-122 and mm-antagomir-122, indicating that silencing was miRNA-specific.

 

 

Cat. #

Products

Quantity

Purification

Price(USD$)

Production Time (Working Days)

B05001

miR-Down Antagomir

2 OD

HPLC

175

10

B05002

miR-Down Antagomir

4 OD

HPLC

250

10

B04007

Antagomir Negative Control Sequence

2 OD

HPLC

175

10

B06001

miR-UP Agomir

2 OD

HPLC

175

10

B06002

miR-UP Agomir

4 OD

HPLC

250

10

B04008

Agomir Negative Control Sequence

2 OD

HPLC

175

10

 
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