Toll-free:400-690-0195ENGLISH简体中文
Search:

RNAi services

Home-Products & services--RNAi services

Product Description

The RNAi technology is not only a powerful weapon for gene function research, but also can be used to inhibit the expression of pathogenic genes, as a potential therapeutic tool for disease treatment. The success of RNAi is dependent on following factors

  • Description
  • Experimental
  • Usage
  • Application
  • Ordering

The RNAi technology is not only a powerful weapon for gene function research, but also can be used to inhibit the expression of pathogenic genes, as a potential therapeutic tool for disease treatment. The success of RNAi is dependent on following factors:

    1. The choice of target sequence and the design of siRNA
    2. Cell line and cell culture system
    3. Transfecion condition
    4. The accumulation and replacement ratio of target gene mRNA
    5. The half life of target protein
    6. The precise analysis in mRNA, Protein and phenotype level

 

The professional technical services of Genepharma’s RNAi service team can help clients achieve the maximal value of RNAi technology and avoid some experimental errors. Our research team will work with you to develop much more extensive and effective solutions.

The RNAi services include two aspects: 

  1. Chemically-synthetic RNAi service;
  2. Vector based RNAi service.

 

GenePharma RNAi service can be applied in following areas:
Functional genomics, gene therapy, drug target screening, cellular signal pathway analysis, protein interaction and so on.

  1. Chemically-synthetic siRNA service
    1. RNAi design: Customer provides target gene name, sequence or GeneBank ID and so on;
    2. siRNA synthesis;
    3. Synthetic siRNA may be further transfected into mammal cells according to customer’s requirement; 
    4. Test of inhibitory effect: the mRNA expression is detected by RT- PCR, the protein expression is detected by Western Blot ;
    5. Provision of experimental reports: siRNA sequence, RT-PCR test report, Western Blot picture and so on. 

 

  1. Vector-based RNAi Service
  2. shRNA design: Customer provides target gene name, sequence or GeneBank ID and so on;
  3. shRNA synthesis;
  4. Choice of shRNA vector:

1.  pGPU6/GFP/Neo
2.  pGPH1/GFP/Neo

  1. Construction of vector;

Constructed vector may be further transfected into mammal cells according to customer’s requirement;

  1. Test of inhibitory effect: the mRNA expression is detected by RT- PCR; the protein expression is detected by Western Blot;
  2. Provision of experimental reports: shRNA sequence, sequencing report of the constructed vector; RT-PCR test report, Western Blot picture and so on. 

Service Fee:
The fee is based on the requirements of the specific experiment.

Reference prices are as follows:

Cat. No.

Service

Price

Times

K-01

siRNA/shRNA Transfecion, RT-PCR ,Western Blot

$ 2,499

4 weeks

K-02

siRNA/shRNA Transfecion, RT- PCR

$ 2,249

4 weeks

K-03

siRNA/shRNA Transfecion, Western Blot 

$ 2,249

4 weeks