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Gene synthesis service

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Product Description

  • Description
  • Experimental
  • Usage
  • Application
  • Ordering

(1) client first need fill in the order form and provide the information of target gene’s name, CDS sequence, gene ID in Gene Bank, and we will provide the quotation to the client. (2) Once confirmed the quotation, GeneParma will sign the service contract and confidentiality agreement with the client, after construct finished, GenePharma will sequence the reconstructed plasmids and ensure the accuracy of the sequence. (3) finally, GenePharma will provide the produts to the customers which include Color sequencing peak figure, the recombinant plasmids inserted the target sequence and the Glycerol broth of E.coli with the recombinant plasmids inside.





Catalog code

Vector name

prompter

Fluorescent label

Nuclear resistence

Prokaryotes
resistence

pEX-1

pGCMV/MCS/EGFP/Neo

CMV

EGFP

Neo

Kan

pEX-2

pGCMV/MCS/IRES/EGFP/Neo

CMV

EGFP

Neo

Kan

pEX-3

pGCMV/MCS/Neo

CMV

--

Neo

Kan

pEX-4

pGCMV/MCS/T2A/EGFP/Neo

CMV

EGFP

Neo

Kan

pEX-5

pGCMV/EGFP/MCS/Neo

CMV

EGFP

Neo

Kan

pEX-6

pGCMV/MCS/RFP/Neo

CMV

RFP

Neo

Kan

pEX-7

pGCMV/RFP/MCS/Neo

CMV

RFP

Neo

Kan
















Ordering
GenePharma Oder Form.xls
The first problem we meet for the application of siRNA is to design the sequences. Although there are quite a lot principles for the designing of published sequences and quite a few companies supply on-line design service, up to now, no software can guarantee the efficiency of the oligos designed.
Shanghai GenePharma Co, Ltd. and our collaboration laboratories have been involved in RNAi research for some time. For each specific gene target, if 4 pairs of oligos were designed and synthesized, normally you will have at least one pair showing 70% down regulation.
Our price: 5OD, PAGE purified, $99/pair
*If one single order is less than 5 pairs extra shipping fee of $40 will be charged. Payment terms are 30 days. We accept cheque or wire transfer into our USD account.
Specifications of siRNA Oligos:
Quality Control:
All our siRNA oligos undergo vigorous process monitoring and strict quality control. Length and labeling are systematically controlled by PAGE or mass spectrometry analysis. Quantity is systematically validated by UV absorbance at 260 nm.
Purification: Fully deprotected and desalted.
Purified by PAGE or RP-HPLC upon request
Length: 19 to 23 mers
Bases: RNA (A, C, G or U)
DNA (A, C, G or T)
Backbone: Phosphodiester bond
Labels and modifications: Fluorescein, biotin and phosphate: 3’ or 5’end
Other labels available upon request
Format: Single-strand RNA oligos is delivered in dry form
Storage and stability
Although oligonucleotides are stable in solution at 4°C for up to 2 weeks, we recommend storage should be at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, siRNA oligos should be dried.
Oligonucleotides with fluorescent labels should be protected from light. We guarantee our oligonucleotides for six months, when stored under the above conditions.
Shipment: Shipped by express delivery, dry in individual, transparent tubes.
Oligonucleotide Technical Data Sheet
Oligonucleotides are delivered with an Oligonucleotide Technical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm , MW, size, extinction coefficient and purification data.
Services available upon request

  1. Aliquoting
  2. Free design support
  3. Additional services may increase turn-around time

Ordering:Please fill in the following order form and send it to: order@genepharma.com or by fax to:00862151320295 our staff will confirm with you asap.