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miRNA RT-PCR detection kits
The Hairpin-itTM miRNAs qPCR Quantitation Kit is a sensitive and specific method using real-time PCR for the detection and quantification of miRNAs (miRNA) from total RNA samples.
 

miRNA RT-PCR Detection Kits

Hairpin-itTM miRNAs qPCR Quantitation Kit
Genepharma’s Hairpin-itTM miRNAs qPCR Quantitation Kit is a sensitive and specific method using real-time PCR for the detection and quantification of micro RNAs (miRNA) from total RNA samples. MiRNAs are small, single-stranded, ~19–23 nucleotide RNA molecules encoded in the genomes of plants, animals, and viruses that play an important role in the control of gene expression at the messenger RNA (mRNA) level. Mature miRNAs enter the RNA-induced silencing complex (RISC) and guide the RISC to induce translational repression or endonucleolytic cleavage of specific target mRNAs. Compared to commonly used methods for detection of miRNAs, the Hairpin-itTM miRNAs qPCR Quantitation Kit is more rapid and sensitive. 
The Kit contains a miRNA-specific RT and PCR primer set, and also includes a Molecular Beacon probe. The special hairpin-like miRNA RT primer and the miRNA-specific Beacon probe ensure the RT and PCR reaction would not be interfered by miRNA precursors. Therefore, the reaction template could be total RNA or even cell lysates.
To normalize for RNA content among different experimental samples, the U6 snRNA qPCR Normalization Kit (Cat # QPM-050) and 5S rRNA qPCR Normalization Kit (Cat # QPM-060) are available separately.
The Hairpin-itTM miRNAs qPCR Quantitation Kit Reagents box includes the following items. Store the components at –20℃. Reagents must be stored in the dark.

Reagent

Amount

Storage

 

100rxns

200rxns

500rxns

 

Real-time PCR Master Mix*

1.0 ml

2×1.0 ml

5×1.0 ml

4℃

miRNA specific primer Set10µM

100μl

200μl

1.0 ml

-20℃

miRNA RT primer10µM

15μl

50μl

100μl

-20℃

Taq DNA polymerase 5U/µl

50μl

100μl

200μl

-20℃

Synthetic miRNA Standard

1nmol

 

 

-20℃

1X RNA Dilution buffer

2×1.0 ml

4×1.0 ml

10×1.0 ml

4℃

*Include dNTP MixtureMg2, Molecular Beacon probe.

 

Accessory Products
Some of the reagents supplied in the Hairpin-itTM miRNAs qPCR Quantitation Kit as well as other products suitable for use with the kit are available separately from GenePharma. Ordering information is provided below.

Item

Amount

Catalog no.

U6 snRNA qPCR Normalization Kit

20 rxns
50 rxns

QPM-090
QPM-091

5S rRNA qPCR Normalization Kit

25μl×50 rxns
25μl×100 rxns

QPM-080
QPM-081

miRNAs qPCR Quantitation Core Kit

100 rxns
200 rxns
500 rxns

QPM-030
QPM-031
QPM-032

Custom miRNAs Quantitation Kit

40µl×50 rxns
40µl×100 rxns
40µl×200 rxns

QPM-040
QPM-041
QPM-042

 
For technical assistance, email support@genepharma.com. For questions related to order placement, email bd@genepharma.com.

 
Description
Application

 

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