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shRNA vector
The siRNA expression vector with antibiotics labeling can continuously suppress the target gene expression, lasting for several weeks or even longer
 

shRNA Vectors

When a validated siRNA sequence (for example, obtained by a large-scale siRNA validation screening) needs to maintain gene silencing for a longer period of time, we recommend the use of a short-hairpin RNA (shRNA) vector system. A shRNA expression vector with an incorporated antibiotic resistance gene can: 1) continuously produce shRNAs inside the cell, and 2) allow for selection of transfected cells. GenePharma is committed to providing the most advanced and convenient shRNA related tools. We recently introduced a new generation highly efficient, ready to use shRNA expression plasmids: our SuperSilencing shRNA Expression Vector System achieves excellent suppression of the target gene, lasting for several weeks or longer.

 

GenePharma SuperSilencing shRNA Expression Vector characteristics:

  • Stable shRNA expression to achieve continuous RNAi.
  • Screening and selectable markers help to establish a stably transfected cell line.  
  • Two cloning restriction sites: BamH I and BbsI. BbsI is a special restriction endonuclease that produces asymmetrical complementary sticky ends to ensure correct insertion of the target DNA oligo, and prevents self-circularization of the plasmid vector.
  • Convenient and easy to operate.
  • Economical: one only needs to synthesize DNA oligos against the target gene.

 

 

 

GenePharma can construct ten kinds of shRNA expression vectors, with or without selectable markers, based on two different plasmids (pGPH1 and pGPU6, see “instructions” and our Catalog for more details on the expression vectors):

pGPU6, pGPH1, pGPU6/Neo, pGPH1/Neo, pGPU6/Hygro, pGPH1/Hygro, pGPU6/GFP/Neo, pGPH1/GFP/Neo, pGPU6/RFP/Neo, pGPH1/RFP/Neo;

pGPU6, pGPH1: shRNA expression plasmids; Selectable and screening markers:

  • Neo: Neomycin (Kan/G418) resistance gene
  • Hygro: Hygromycin B resistance gene
  • GFP: GFP reporter gene
  • RFP: RFP reporter gene

 

GenePharma SuperSilencing shRNA Expression Vector Set
Each set contains 4 linear or cyclic shRNA expression vectors designed for stable RNAi knockdown of your target gene: we guarantee that at least one of them causes at least 70% inhibition efficiency at the mRNA level. Each set also includes the corresponding negative control vector and a GFP positive control vector. Additionally, we provide you with the overall solution.

 

Product

Price (USD$)

Production Time

SuperSilencing shRNA Expression Vector Set (Cat. #C01001)

$650

2 weeks


Composition

Amount (µg)

4x shRNA target gene vector

4 x 50

1x positive control shRNA vector

1 x 50

1x negative control shRNA vector

1 x 50

TOTAL                                                                                        6 x 50


Cat. No.

Product

Promoter

Selection marker

Quantity

Price

E-01

pGPU6

U6

6*50μg

$650

E-02

pGPH1

H1

6*50μg

$650

E-03

pGPU6/Neo

U6

Neo

6*50μg

$650

E-04

pGPH1/Neo

H1

Neo

6*50μg

$650

E-05

pGPU6/Hygro

U6

Hygro

6*50μg

$650

E-06

pGPH1/Hygro

H1

Hygro

6*50μg

$650

E-07

pGPU6/GFP/Neo

U6

Neo

6*50μg

$650

E-08

pGPH1/GFP/Neo

H1

Neo

6*50μg

$650

E-09

pGPU6/RFP/Neo

U6

Neo

6*50μg

$650

E-10

pGPH1/RFP/Neo

H1

Neo

6*50μg

$650

 

GenePharma ready-to-use SuperSilencing shRNA Expression Vector Cloning and Construction Service 
We can help you to insert the DNA fragment encoding target shRNA into the plasmid and do sequence validation. All we need is the target gene sequence or Gene ID and your plasmid vector system of choice, and we will help you to construct the expression vector. Furthermore, we will provide you with sufficient purified expression plasmid encoding your target shRNA. 

Cat. No.

Product

Promoter

Selectable Marker

Quantity

Price

Production Time

C02001

pGPU6

U6

50μg

$149

2 weeks

C02002

pGPH1

H1

50μg

$149

2 weeks

C02003

pGPU6/Neo

U6

Neo

50μg

$149

2 weeks

C02004

pGPH1/Neo

H1

Neo

50μg

$149

2 weeks

C02005

pGPU6/Hygro

U6

Hygro

50μg

$149

2 weeks

C02006

pGPH1/Hygro

H1

Hygro

50μg

$149

2 weeks

C02007

pGPU6/GFP/Neo

U6

Neo

50μg

$149

2 weeks

C02008

pGPH1/GFP/Neo

H1

Neo

50μg

$149

2 weeks

C02009

pGPU6/RFP/Neo

U6

Neo

50μg

$149

2 weeks

C02010

pGPH1/RFP/Neo

H1

Neo

50μg

$149

2 weeks

For technical assistance, email support@genepharma.com. For questions related to order placement, email bd@genepharma.com.

 
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